Protein Engineering of Detergent .ALPHA.-Amylases
نویسندگان
چکیده
منابع مشابه
Protein engineering of bacterial K-amylases
K-Amylases constitute a very diverse family of glycosyl hydrolases that cleave K1C4 linkages in amylose and related polymers. Recent structural and mutagenic studies of archeael, mammalian and bacterial K-amylases have resulted in a wealth of information on the catalytic mechanism and on the structural features of this enzyme class. Because of their high thermo-stability, the Bacillus K-amylase...
متن کاملCharacteristics of two forms of alpha-amylases and structural implication.
Complete (Ba-L) and truncated (Ba-S) forms of alpha-amylases from Bacillus subtilis X-23 were purified, and the amino- and carboxyl-terminal amino acid sequences of Ba-L and Ba-S were determined. The amino acid sequence deduced from the nucleotide sequence of the alpha-amylase gene indicated that Ba-S was produced from Ba-L by truncation of the 186 amino acid residues at the carboxyl-terminal r...
متن کاملAmylases: an Overview with Special Reference to Alpha Amylase
1Department of Microbiology, VBS Purvanchal University, Jaunpur, UP, India 2Department of Environmental Sciences, VBS Purvanchal University, Jaunpur, UP, India 3Department of Microbiology, HNB Garhwal University, Srinagar, UK, India 4 Department of Biological Sciences, MGCG University, Satna, MP, India 5 Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India 6Dep...
متن کاملalpha-Amylases and approaches leading to their enhanced stability.
The recent state of the knowledge of properties and structure of alpha-amylases is reviewed with the aim of elucidation the basis for their stabilization. Three principal ways for obtaining stable alpha-amylases (isolation of enzymes from extremophiles, production of extremophilic enzymes in mesophiles, and modification of mesophilic enzymes) are discussed separately. Detailed experimental exam...
متن کاملSubsite mapping of enzymes. Application of the depolymerase computer model to two alpha-amylases.
In the preceding paper (Allen and Thoma, 1976) we developed a depolymerase computer model, which uses a minimization routine to establish a subsite map for a depolymerase. In the present paper we show how the model is applied to experimental data for two alpha-amylases. Michaelis parameters and bond-cleavage frequencies for substrates of chain lengths up to twelve glucosyl units have been repor...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2003
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.15.101